popPCR

Classify Digital PCR Droplets by Fitting Fluorescence Populations

CRAN Package

Estimates DNA target concentration by classifying digital PCR (polymerase chain reaction) droplets as positive, negative, or rain, using Expectation-Maximization Clustering. The fitting is accomplished using the 'EMMIXskew' R package (v. 1.0.3) by Kui Wang, Angus Ng, and Geoff McLachlan (2018) as based on their paper "Multivariate Skew t Mixture Models: Applications to Fluorescence-Activated Cell Sorting Data" <doi:10.1109/DICTA.2009.88>.

  • Version0.1.1.1
  • R versionunknown
  • LicenseGPL (≥ 3)
  • Needs compilation?Yes
  • Last release03/09/2021

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