TmCalculator

This tool is extended from methods in Bio.SeqUtils.MeltingTemp of python. The melting temperature of nucleic acid sequences can be calculated in three method, the Wallace rule (Thein & Wallace (1986)), empirical formulas based on G and C content (Marmur J. (1962), Schildkraut C. (2010), Wetmur J G (1991), Untergasser,A. (2012), von Ahsen N (2001)) and nearest neighbor thermodynamics (Breslauer K J (1986), Sugimoto N (1996), Allawi H (1998), SantaLucia J (2004), Freier S (1986), Xia T (1998), Chen JL (2012), Bommarito S (2000), Turner D H (2010), Sugimoto N (1995), Allawi H T (1997), Santalucia N (2005)), and it can also be corrected with salt ions and chemical compound (SantaLucia J (1996), SantaLucia J(1998), Owczarzy R (2004), Owczarzy R (2008)).

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